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Pusztai's Research under Official Discussion

 
Claim: Anti-genetic engineering campaigners have been using the controversial results of Arpad Pusztai's feeding studies in rats with genetically modified potatoes as a key argument in their battle against gene technology. In his hitherto unpublished study Pusztai concluded that the vector used to transplant new genes (a basic tool of plant breeders who employ biotechnological methods) may have been responsible for severe organ and brain damage and compromised immune defences in his rats. Pusztai's data have now been published in The Lancet, the prestigious British medical journal, in an article entitled: Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine.

The facts: The controversy surrounding the study meant that decision of the editor of the Lancet to publish the data was not an easy one. One of the peer reviewers who requested that his name be kept secret said it was wrong to publish the study as Pusztai's conclusions were wild speculations. A statistics specialist - also involved in the peer review process - added that the statistical methods employed were inadequate and the analyses quite simply wrong. Another scientist, Martin Chrispeels, agreed: " This isn't science. It wouldn't be published in a serious plant biology journal. Their conclusion is not correct." Another of the six peer reviewers - usually there are only two or three - John Pickett, even went on public record to say it would be a very sad day if such a respected journal were to ignore the advice of its own peer reviewers.
Why, then, did Richard Horton, editor of The Lancet, go ahead with publication against his reviewers' recommendation? He says why in the same edition under the heading "Genetically modified foods: "absurd" concern or welcome dialogue?". His perspective is the viewpoint of the concerned (British) public. He argues that " risks are not simply questions of abstract probabilities or theoretical reassurances. What matters is what people believe about these risks and why they hold those beliefs."

Horton sees the gap between science and society getting wider and, in the post-BSE age, has hit the nail squarely on the head with this assessment. Science is under growing pressure to legitimate itself and is facing an enormous communication deficit which it studiously ignores and appears unequal to deal with. Horton backs up his views with statements from representatives of industry and science who have eyes only for the fact that biotechnology in Europe is lagging behind and tend to attribute public concerns to pure ignorance. However, Horton also insinuates that he shares his reviewers' opinion of the scientific reliability of Pusztai's data. Nevertheless he pleads for debate, saying that forever attacking the critics instead of including them in the discussion process will only increase public mistrust in scientists and science.
In spite of the earlier mentioned but obviously non-existing symptoms and signs of the rats' diseased state - they had neither brain nor liver damage, nor were they immune suppressed - Pusztai and Ewen nevertheless come to the conclusion that the differences in small intestine histology in the rat groups on different diets (potatoes with added GNA and GNA-lectin-producing potatoes) could be the results of transformation of the potatoes with the GNA gene. But the data are completely inconsistent. One time Pusztai observes an effect in a comparison of the cooked potatoes, another time in the raw potatoes.
This seems to be a case of too many effects getting on top of each other. Harry Kuiper points out an elementary methodological error in his article entitled "Adequacy of methods for testing the safety of genetically modified foods" published in the same issue of The Lancet. He rightly criticizes the fact that Pusztai and Ewen failed to discuss the possible differences in composition (macronutrients and micronutrients, toxins) of the transgenic and parent potato lines - whereas, as Kuiper states, " Pusztai has released some of these details on the internet.

These details indicate that the content of starch, glucose polymers, lectin, and trypsin and chymotrypsin inhibitors in GM potatoes differed from that of the parental line." Pusztai himself therefore provides evidence of the lack of substantial equivalence of the two lines and, by the same token, of the lack of comparability in his feeding studies. Another problematic issue is the excessively low protein content in the potatoes of only 6%. A healthy diet should contain approximately 15% protein.

On balance, too many effects and uncertainties cloud the sparse data, the interpretation of which necessarily enters the realm of speculation. To finish, Pusztai uses his publication as a forum for general criticism and uses his data as a basis for making a sweeping generalization in respect of transgenic plants in general: The possibility that a plant vector in common use in some GM plants can ... exert powerful biological effects may also apply to GM plants containing similar constructs, particularly those containing lectins, such as soy beans. This statement - RR-soybeans are cultivated on millions of hectares in the USA - is one that Pusztai certainly cannot support from his weak data.

His comments are not just speculative, they are unscientific to a high degree.Kuiper also discusses the safety of transgenic plants in his article and reaches a more discerning conclusion, i. e. concludes that the concept of substantial equivalence is an adequate principle on the basis of which to test and demonstrate the safety of today's generation of transgenic plants. He identifies a need for action as regards future developments, however, stating that the second generation of transgenic plants whose product quality has been optimized in terms of protein or fatty acid composition also necessitates adaptation of safety tests and development of new methods.

A fourth article in the same edition of The Lancet criticizes Pusztai's basic idea of integrating Galanthus nivalis lectin (GNA) into plants as a natural insecticide. Brian Fenton from the Scottish Crop Research Institute - the institute Pusztai cooperated with - describes the interaction of GNA and human cells as being inadequately understood. He and his colleagues show in an experiment that - in contrast to Pusztai's claims - GNA binds to human white blood cells and thus has the potential to trigger biological effects. This is a very subtle criticism of Pusztai's work, as the latter assumed that GNA - unlike other lectins - is not toxic to the mammalian organism, which is why he chose precisely this lectin for transformation of his potatoes.
In publishing Pusztai's data, The Lancet afforded him the opportunity of officially presenting his findings. However, the printed paper is unscientific and in no way calls the safety of transgenic plants into question. Nor does it go any way towards rehabilitating Pusztai. Pusztai's data no longer need to be discussed on the quiet and nobody can claim that information is being suppressed. The Lancet has certainly taken a bold and unusual step, but lives up to its reputation for good science by the simultaneous publication of another three articles on the topic. Why shouldn't a scientific journal take on the task of pointing out blatant deficits in communication and drawing attention to the dwindling public trust in its country's scientists?
 

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